Anopheles coluzzii strain N’Gousso, with custom gene silencing, infected with Plasmodium falciparum

0.00

Reference: V.2.1.1.I1.S.DE.141.25
Origin: Yaoundé, Cameroon, 2006
Material provided: Whole mosquitoes, dissected tissues, or extracts
Unit definition: 50 gene-silenced infected females (25 if dissected)
Provider country: Germany (Max Planck Institute for Infection Biology - MPIIB)

Specific dsRNA will be provided by the end-user. A. coluzzii Ngousso mosquitoes are microinjected with specific dsRNA at requested time before infection.

SKU: V.2.1.1.I1.S.DE.141.25 Category: Tags: ,

Description

Material provided: Whole mosquitoes, dissected tissues, or extracts
Unit definition: 50 infected females (25 if dissected) supplied as 100 fully-engorged females with infection prevalence at least 50%

Vector information

Family: Culicidae
Genus: Anopheles
Species: Anopheles coluzzii
Strain Name: N‘Gousso
Place of origin: Yaoundé, Cameroon
Date of colonisation: 2006
Genotype features TEP1*S1
Phenotype features:  susceptible to P. falciparum

Parasite information

Phylum: Apicomplexa
Genus: Plasmodium
Species: Plasmodium falciparum
Strain: Plasmodium falciparum isolate NF54 was originally isolated from an individual living near Schiphol airport, Amsterdam, The Netherlands.
Infectivity: Infection at 0.3% final gametocytaemia gives generally 40,000-80,000 spz / mosquito 14 days post infection.
Mycoplasmic content: Asexual cultures are regularly checked for Mycoplasma contamination.

Production protocol

Specific dsRNA will be provided by the end-user. A. coluzzii Ngousso mosquitoes are microinjected with specific dsRNA at requested time before infection. Injected mosquitoes are fed through membrane feeders at 37°C for 15 min on the NF54 gametocyte cultures with a final gametocytaemia ranging between 0.15 to 0.30%. Unfed mosquitoes are removed after feeding. Infected mosquitoes are kept thereafter in the secured S3 laboratory at 26°C for 1-15 days before dissection.

Please specify in your request:

Time post-infection: Please specify one time point post-infection to harvest the samples. The most common options are ookinete infection (18-24 h post-infection), oocyst infection (2-13 d post-infection) and sporozoite infection (14-15 d post-infection), but other times are possible by request, contact us.

Whole or dissected: Instead of 50 whole mosquitoes, material can be supplied from dissected material harvested at the appropriate time point: 25 dissected midguts or 25 dissected salivary glands or 25 dissected ovaries, with the corresponding carcasses.

Form of material:

  • Whole mosquitoes or dissected tissues, fixed in 70% ethanol, formalin, or other fixative: useful for DNA extraction from 70% ethanol, immunohistochemistry, not useful for RNA experiments or proteomics. Shipped as non-infectious material.
  • Whole mosquitoes or dissected tissues, homogenised in a lysis buffer such as Trizol: useful for RNA, DNA and protein experiments. Shipped as non-infectious material.
  • Whole mosquitoes or dissected tissues, frozen at -80°C or stored in RNALater: useful for RNA, DNA and protein experiments. Shipped as non-infectious material.
  • Other formats or preparations may be possible by request, contact us.

Production conditions: Material is generated using default gametocyte dose and incubation temperature used by the supplying facility. Other conditions may be possible upon request.

Sample size and replicates: For larger sample sizes, request more units. Please specify if independent infections are needed as biological replicates (e.g., for RNAseq transcriptional profiling experiments).

Publications

  • Harris, C., Lambrechts, L., Rousset, F., Abate, L., Nsango, S.E., Fontenille, D., Morlais, I., and Cohuet, A. (2010). Polymorphisms in Anopheles gambiae immune genes associated with natural resistance to Plasmodium falciparum. PLoS Pathog 6, e1001112.
  • Ponnudurai, T., Leeuwenberg, A. D. and Meuwissen, J. H. (1981) Chloroquine sensitivity of isolates of Plasmodium falciparum adapted to in vitro culture. Tropical and Geographical Medicine 33, 50-54.

For more information, please contact us.

Additional information

Provider country

Germany

Provider(s)

Max Planck Institute for Infection Biology (MPIIB)

Eligibility

In order to conform to H2020 rules promoting scientific interaction between countries, if your institute is located in the provider country, you need to choose another similar product, or form a user group to request this product, Please check: http://infravec2.eu/user-groups/