Culex pipiens UAB strain, infected with West Nile virus

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Reference: V.3.1.4.I6.SP.44.9
Origin: Spain
Material provided: Whole mosquitoes, dissected tissues, or extracts
Unit definition: 50 infected females
Provider country: Spain (Institut de Recerca i Tecnologia Agroalimentàries - IRTA)

User field-collected strain is sent to the Provider. 7-9 days old females are fed on an infectious blood-meal or intra-thoracically inoculated.

SKU: V.3.1.4.I6.SP.44.9 Category: Tag:

Description

Material provided: Whole mosquitoes, dissected tissues, or extracts
Unit definition: 50 infected females
Description: User field-collected strain is sent to the Provider. 7-9 days old females are fed on an infectious blood-meal or intra-thoracically inoculated.

Vector information

Family: Culicidae
Genus: Culex
Species: Culex pipiens
Strain Name: UAB strain
Place of origin: Spain
Date of colonisation: 2012

Virus information

Pathogens used for infection: West Nile virus
Reference: WNV
ICTV Taxonomy: ssRNA+, Flaviviridae
Virus name: West Nile virus
Sequencing: partial sequence
Infectivity: Infectivity tested and quantified
Mycoplasmic content: Mycoplasma free

Production protocol

User field-collected strain is sent to the Provider. 7-9 days old females are fed on an infectious blood-meal using the Hemotek® system or intra-thoracically inoculated. The titer of infectious blood-meals is up to 107 TCID50/mL. Exposed females are transferred to small containers and fed with 10% sucrose in a chamber under controlled environmental conditions (temperature, light: dark cycle and humidity) until maximum day 14 post-exposure.

Product options

Please specify in your request:

Time post-infection: Please specify one time point in days post-infection (dpi) to harvest the samples. The most common options are primary midgut infection (3 dpi) or disseminated infection (7 or 14 dpi), but other times are possible by request, contact us.

Whole or dissected: Instead of 50 whole mosquitoes, material can be supplied from dissected material harvested at the appropriate time point: 25 dissected midguts or 25 dissected salivary glands or 25 dissected ovaries, provided with the corresponding carcasses.

Form of material: Whole mosquitoes or dissected tissues, fixed in formalin, or other fixative validated for virus inactivation: useful for DNA extraction, immunohistochemistry, not useful for RNA experiments or proteomics. Shipped as non-infectious material.

  • Whole mosquitoes or dissected tissues, homogenised in a lysis buffer such as Trizol validated for virus inactivation: useful for RNA, DNA and protein experiments. Shipped as non-infectious material.
  • Whole mosquitoes or dissected tissues, frozen at -80°C or stored in RNALater that do not efficiently inactivate virus: useful for RNA, DNA and protein experiments. Shipped as infectious material.
  • Other formats or preparations may be possible by request, contact us.

Production conditions: Material is generated using default virus dose and incubation temperature used by the supplying facility. Other conditions may be possible upon request, contact us.

Sample size and replicates: For larger sample sizes, request more units. If requesting multiple units, please clearly specify the purpose (biological replicates, larger sample size, or other). Biological replicates (e.g., for RNAseq transcriptional profiling experiments) require independent infections, while a larger sample size of the same material requires infecting additional mosquitoes on the same infectious blood.

For more information, please contact us.

Additional information

Provider country

Spain

Provider(s)

IRTA – CReSA

Eligibility

In order to conform to H2020 rules promoting scientific interaction between countries, if your institute is located in the provider country, you need to choose another similar product, or form a user group to request this product, Please check: http://infravec2.eu/user-groups/