Mosquito experimental infections with CL2 arboviruses

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Reference: F.9.NC.137.19
Material provided: Physical access for end-users
Unit definition: 1 week of access
Provider country: New Caledonia (Institut Pasteur de Nouvelle Calédonie - IPNC)

Equipped CL2 lab to undertake experimental infections on Aedes aegypti from New Caledonia with arboviruses: dengue, chikungunya or Zika.

Description

Material provided: Physical access for end-users
Unit definition: 1 week of access
Location: Institut Pasteur de Nouvelle Calédonie (IPNC), Noumea, New Caledonia
Biosafety Level: CL2
Installation name: P2+ Lab at IPNC
Website: IPNC

The facility

The P2+ Lab is located at IPNC, Noumea, New Caledonia. It is a biosecurity lab dedicated to the study of pathogens. IPNC has got authorization to conduct research on endogenous vectors and arboviruses. IPNC has got CL1 insectary (30 m2) with two rooms dedicated to insect rearing and insecticide resistance surveys. The P2+ lab has two rooms dedicated to arboviruses production and experimental infection assays (25 m2).
IPNC hosts the lab in charge of entomological surveillance in New Caledonia. IPNC is involved in arboviruses surveillance in New Caledonia.

Available vectors

Mosquito population to be used: Aedes aegypti from New Caledonia from field-collection or from lab colony (susceptible/resistant to deltamethrin).

Services offered

One Unit is a 1-week access to a dedicated CL2 lab to conduct experimental infections on Ae. aegypti from New Caledonia
The unit of access offers the rearing of the mosquitoes necessary for the experiments prior to the arrival of the user and preparation virus stock. Experimental infection with arboviruses (dengue, Zika or chikungunya) and subsequent analysis will be realised by the user with help from a technician and supervision by a researcher for completion of the assays when necessary.

Equipment

  • Biological Safety cabinet, type 2, Safe Fast Elite
  • CO2 incubator, IGO150, Jouan
  • Incubator, UM100, Memmert
  • Climate Chamber, HPP260, Memmert
  • Inverted Microscope, DMIL, Leica
  • Refrigerated centrifuge, 216PK, Sigma
  • -20-degree freezer and refrigerator
  • Waterbath, Polytest 8, Bioblock
  • Refrigerated table
  • Artificial feeding equipment, Hemotek

For more information, please contact us.

Additional information

Provider country

New Caledonia

Provider(s)

Pasteur Institute New Caledonia (IPNC)

Additional conditions

For physical access to Infravec2 facilities at Biosafety Level 2 or 3, please provide evidence of training and authorization, If needed, you may apply for our training courses: : please contact tnamanager@infravec2.fr

Travel and lodging

A fixed amount for travelling and lodging costs is included in the physical access grant

Eligibility

In order to conform to H2020 rules promoting scientific interaction between countries, if your institute is located in the provider country, you need to choose another similar product, or form a user group to request this product, Please check: http://infravec2.eu/user-groups/