Aedes aegypti strain PAEA preserved or extracts

0.00

Reference: V.1.1.1.P.FR.4.2
Origin: Paea, Tahiti (French Polynesia), 1994
Material provided: Whole mosquitoes, dissected tissues, or extracts
Unit definition: 100 mosquitoes (50 if dissected)
Provider country: France (Institut Pasteur - IP)

Eggs are hatched in dechlorinated tap water and larvae are reared under controlled conditions.

SKU: V.1.1.1.P.FR.4.2 Category: Tags: ,

Description

Material provided: Whole mosquitoes, dissected tissues, or extracts
Unit definition: 100 mosquitoes (50 if dissected)

Vector information

Family: Culicidae
Genus: Aedes
Species: Aedes aegypti
Strain Name: PAEA
Place of origin: Paea, Tahiti (French Polynesia)
Date of colonisation: 1994

Production protocol

Eggs are hatched in dechlorinated tap water and larvae are reared under controlled conditions of 150–200 larvae per 1 liter and fed with one yeast tablet renewed every 3–4 days. Adults are kept in cages at 28°±1°C with a 16h: 8h light: dark cycle, 80% relative humidity, and supplied with a 10% sucrose solution.

Product options

Please specify in your request:

Sample size: For larger sample sizes, request more units.

Whole or dissected: 100 whole mosquitoes; or 50 dissected midguts, salivary glands or ovaries

Form of material:

  • Whole mosquitoes or dissected tissues, fixed in 70% ethanol, formalin, or other fixative: useful for DNA extraction from 70% ethanol, immunohistochemistry, not useful for RNA experiments or proteomics.
  • Whole mosquitoes or dissected tissues, homogenised in a lysis buffer such as Trizol: useful for RNA, DNA and protein experiments.
  • Whole mosquitoes or dissected tissues, frozen at -80°C or stored in RNALater useful for RNA, DNA and protein experiments.
  • Other formats or preparations may be possible by request, contact us.

Publications

  • Vazeille-Falcoz M, Mousson L, Rodhain F, Chungue E, Failloux AB. 1999. Variation in oral susceptibility to dengue type 2 virus of populations of Aedes aegypti from the islands of Tahiti and Moorea, French Polynesia. American Journal of Tropical Medicine and Hygiene 60: 292-299.

For more information, please contact us

Additional information

Provider country

France

Provider(s)

Pasteur Institute Paris (IPP)

Eligibility

In order to conform to H2020 rules promoting scientific interaction between countries, if your institute is located in the provider country, you need to choose another similar product, or form a user group to request this product, Please check: http://infravec2.eu/user-groups/

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