Vector Research Products


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Type of service

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Long-read DNA sequencing by Nanopore with bioinformatic analysis

Material provided: Data, including bioinformatic analysis
Unit definition: 10 samples/run
Description : Long-read sequencing technologies are capable of producing long sequencing reads with average fragment lengths of over 10,000 base-pairs and maximum lengths reaching 100,000 base- pairs. Bioinformatic analysis.

Long-read DNA sequencing by Nanopore

Material provided: Data (raw sequence files)
Unit definition: 10 samples/run
Description : Long-read sequencing technologies are capable of producing long sequencing reads with average fragment lengths of over 10,000 base-pairs and maximum lengths reaching 100,000 base- pairs.

Small RNA Illumina sequencing with bioinformatic analysis

Material provided: Data. Bioinformatic analysis included
Unit definition: 12 samples/run
Description : Small RNA sequencing (RNA-Seq) is a technique to isolate and sequence small RNA species: miRNAs, siRNAs, piRNAs, and snoRNAs with a size range of 20 to 30 nucleotides that play a crucial role in regulating gene expression. Bioinformatic analysis.

Small RNA Illumina sequencing

Material provided: Data (raw sequence files)
Unit definition: 12 samples/run
Description : Small RNA sequencing (RNA-Seq) is a technique to isolate and sequence small RNA species: miRNAs, siRNAs, piRNAs, and snoRNAs with a size range of 20 to 30 nucleotides that play a crucial role in regulating gene expression.

Metagenomic Illumina sequencing with bioinformatics analysis

Material provided: Data, bioinformatics analysis included
Unit definition: 24 samples/run
Description : The v3-v4 regions of the 16S rRNA gene will be amplified and sequenced on an Illumina MiSeq instrument using 250 paired – end protocol. 16S data processing to remove low quality reads, specifically reads with average Phred quality less than 20 over 30-bp window […]

Metagenomic Illumina sequencing of 16S rRNA gene amplicons

Material provided: Data (raw sequence files)
Unit definition: 24 samples/run
Description : Sequence variation in the prokaryotic 16S ribosomal RNA gene (16S rRNA) is widely used to characterize microbial communities. The v3-v4 regions of the 16S rRNA gene will be amplified and sequenced on an Illumina MiSeq instrument using 250 paired – end protocol.

DNA Illumina sequencing with bioinformatics analysis

Material provided: Data (raw sequence files), bioinformatic analysis included
Unit definition: 16 samples/lane for Whole Genome Sequencing; 32/samples run for Amplicon Based Sequencing
Description : Whole genome sequencing or amplicon-based sequencing allow, respectively, the analysis of the entire genome or of specific sections by amplicons. Assembling & single nucleotide variations bioinformatics analysis to analyse the entire genome or specific pieces by amplicons.

DNA Illumina sequencing (whole genome or amplicon-based sequencing)

Material provided: Data (raw sequence files)
Unit definition: 16 samples/lane for Whole Genome Sequencing; 32/samples run for Amplicon Based Sequencing
Description : Whole genome sequencing or amplicon-based sequencing allow, respectively, the analysis of the entire genome or of specific sections by amplicons.

Analysis of cuticle thickness and epicuticular lipid content, as a marker of insecticide resistance in mosquito vectors

Material provided: Summary report on cuticle thickness determination and levels of lipids
Unit definition: Data for 20-40 specimens/population or strain
Description : We employ specialized Transmission Electron Microscopy (TEM) - based determinations of cuticle thickness to determine the presence of the trait in mosquito samples

Assess the metabolic liability and potential of novel insecticidal active ingredients (chemicals, plant extracts, biopesticides, etc) to break insecticide resistance

Material provided: Summary report on the metabolic liability and efficiency of novel compounds against highly resistant lines
Unit definition: A complete screen against >5 detoxification enzymes and >5 drosophila/insect strains
Description : In vitro and in vivo evaluation assays for screening putative novel active ingredients, to predict their metabolic liability and potential to break insecticide resistance.

Genotyping of mosquitoes for species ID, Insecticide Resistance traits (target site mutations and detoxification genes) and detection of plasmodium, arboviruses and/or other pathogens

Material provided: Summary report on mosquito genotyping and virus/pathogen detection
Unit definition: Genotyping of one mosquito sample (20-50 female mosquitoes) × 20-30 markers
Description : By using RNAlater preserved samples, and either multiplex Taqman assays OR our novel Labdisk platform, we provide genotyping for approximately 20-30 most relevant to vector control markers.

Aedes albopictus - User strain - infected with Chikungunya virus

Material provided: Fixed whole or partial mosquito samples
Unit definition: Non-infectious material derived from 50 infected females
Description : 1-week-old females are fed on an infectious blood-meal using the Hemotek® system.

Aedes albopictus - User strain - infected with dengue virus

Material provided: Fixed whole or partial mosquito samples
Unit definition: Non-infectious material derived from 50 infected females
Description : 1-week-old females are fed on an infectious blood-meal using the Hemotek® system.

Aedes albopictus - User strain - infected with Zika virus

Material provided: Fixed whole or partial mosquito samples
Unit definition: Non-infectious material derived from 50 infected females
Description : 1-week-old females are fed on an infectious blood-meal using the Hemotek® system.

Aedes albopictus strain Rimini infected with Chikungunya virus

Place of origin: Rimini, Italy, 2004
Material provided: Fixed sample or extract
Unit definition: 50 infected females
Description : Rearing rooms maintained at 25°C ± 1°C and 16:8 light:dark cycle […]

Aedes albopictus strain Rimini infected with dengue virus

Place of origin: Rimini, Italy, 2004
Material provided: Non infectious material
Unit definition: Extract or fixed sample derived from 50 infected females
Description : Rearing rooms maintained at 25°C ± 1°C and 16:8 light:dark cycle […]

Aedes albopictus strain Rimini infected with Zika virus

Place of origin: Rimini, Italy, 2004
Material provided: Fixed sample or extract
Unit definition: 50 infected females
Description : Rearing rooms maintained at 25°C ± 1°C and 16:8 light:dark cycle […]

Culex pipiens strain Gavà, infected with Usutu virus

Place of origin: Gavà, Catalonia, Spain, 2012
Material provided: Fixed sample or extract
Unit definition: 50 infected females
Description : 10-12 days old females are fed on an infectious blood-meal using the Hemotek® system or intra-thoracically inoculated. […]

Culex quinquefasciatus wild-collected preserved specimens, New Caledonia

Place of origin: New Caledonia
Material provided: Fixed sample or extract
Unit definition: 100 preserved specimens
Description : Wild larvae or adult mosquitoes will be collected in New Caledonia.

Aedes albopictus strain Rimini preserved specimens

Place of origin: Rimini, Italy, 2004
Material provided: Fixed sample or extract
Unit definition: 50 preserved specimens
Description : Rearing rooms maintained at 25°C ± 1°C and 16:8 light:dark cycle […]

Aedes albopictus strain Mauritius-2013 infected with Chikungunya virus

Place of origin: Mauritius island, 2013
Material provided: Fixed sample or extract
Unit definition: 50 infected females
Description : Rearing rooms maintained at 25°C ± 1°C and 16:8 light:dark cycle […]

Aedes albopictus strain Mauritius-2013 infected with dengue virus

Place of origin: Mauritius island, 2013
Material provided: Fixed sample or extract
Unit definition: 50 infected females
Description : Rearing rooms maintained at 25°C ± 1°C and 16:8 light:dark cycle […]

Aedes albopictus strain Mauritius-2013 infected with Zika virus

Place of origin: Mauritius island, 2013
Material provided: Fixed sample or extract
Unit definition: 50 infected females
Description : Rearing rooms maintained at 25°C ± 1°C and 16:8 light:dark cycle […]

Aedes albopictus strain Mauritius-2013 preserved specimens

Place of origin: Mauritius, 2013
Material provided: Fixed sample or extract
Unit definition: 50 preserved specimens
Description : Rearing rooms maintained at 25°C ± 1°C and 16:8 light:dark cycle […]

Anopheles stephensi strain Sind-Kasur Nijmegen infected with Plasmodium falciparum GFP-Luciferase (HSP promoter)

Place of origin: Pakistan, 1982
Material provided: Fixed sample or extract
Unit definition: 50 infected females
Description : A P. falciparum NF54-CGL stage V gametocytes culture with 5x105 exflagellation events / ml is used for feeding of female Anopheles stephensi mosquitoes. […]

Anopheles stephensi strain Sind-Kasur Nijmegen infected with Plasmodium falciparum GFP-Luciferase (CSP promoter)

Place of origin: Pakistan, 1982
Material provided: Fixed sample or extract
Unit definition: 50 infected females
Description : A P. falciparum NF54-CGL stage V gametocytes culture with 5x105 exflagellation events / ml is used for feeding of female Anopheles stephensi mosquitoes. […]

Anopheles stephensi strain Sind-Kasur Nijmegen infected with Plasmodium falciparum

Place of origin: Pakistan, 1982
Material provided: Live or fixed mosquitoes
Unit definition: 200 infected females
Description : A P. falciparum NF54 stage V gametocytes culture with 5x105 exflagellation events / ml is used for feeding of female Anopheles stephensi mosquitoes […]

Aedes aegypti custom transgenic line

Place of origin: Bangkok PhiC31 docking line number 12
Material provided: Dried eggs
Unit definition: 1 mosquito transgenic line (batch of 1000 eggs)
Description : Mosquito transgenic line

Aedes aegypti strain ir0115 (French Guiana) infected with Chikungunya virus

Place of origin: Ile Royale (French Guiana), 2015
Material provided: Fixed sample or extract
Unit definition: 50 infected females
Description : Chikungunya virus maintained in C6/36 cells. Hemotek feeding within rabbit blood.

Aedes aegypti strain ir0115 (French Guiana) preserved specimens

Place of origin: Ile Royale (French Guiana), 2015
Material provided: Fixed sample or extract
Unit definition: 50 preserved specimens
Description : Ae. aegypti conventional rearing. Dissection in PBS.

Aedes aegypti strain ir0115 (French Guiana) live eggs

Place of origin: Ile Royale (French Guiana), 2015
Material provided: Live eggs
Unit definition: Batch of >5000 live eggs
Description : Conventional Ae. aegypti rearing.

Phlebotomus duboscqi infected with Leishmania species

Place of origin: Senegal, before 1994
Material provided: Fixed sample or extract
Unit definition: 100 infected specimens
Description : 3-5 day old females are fed through chick-skin membrane on the mixture of heat inactivated rabbit blood and Leishmania promastigotes [...]

Sergentomyia schwetzi B live adults

Place of origin: Ethiopia, 2010
Material provided: Live adults
Unit definition: Batch of 200 live adults
Description : Laboratory colony maintained under standard conditions at 26±1 °C, 90% relative humidity and 12/12 light/dark photoperiod. [...]

Sergentomyia schwetzi A live adults

Place of origin: Ethiopia, 2010
Material provided: Live adults
Unit definition: Batch of 200 live adults
Description : Laboratory colony maintained under standard conditions at 26±1 °C, 90% relative humidity and 12/12 light/dark photoperiod. [...]

Sergentomyia schwetzi B infected with Leishmania species

Place of origin: Ethiopia, 2010
Material provided: Fixed sample or extract
Unit definition: 100 infected specimens
Description : 3-5 day old females are fed through chick-skin membrane on the mixture of heat inactivated rabbit blood and Leishmania promastigotes [...]

Sergentomyia schwetzi A infected with Leishmania species

Place of origin: Ethiopia, 2010
Material provided: Fixed sample or extract
Unit definition: 100 infected specimens
Description : 3-5 day old females are fed through chick-skin membrane on the mixture of heat inactivated rabbit blood and Leishmania promastigotes [...]

Phlebotomus tobbi live adults

Place of origin: Turkey, 2008
Material provided: Live adults
Unit definition: Batch of 200 live adults
Description : Laboratory colony maintained under standard conditions at 26±1 °C, 90% relative humidity and 12/12 light/dark photoperiod. [...]

Phlebotomus sergenti live adults

Place of origin: Turkey, before 2005
Material provided: Live adults
Unit definition: Batch of 200 live adults
Description : Laboratory colony maintained under standard conditions at 26±1 °C, 90% relative humidity and 12/12 light/dark photoperiod. [...]

Phlebotomus perniciosus live adults

Place of origin: Spain, before 1994
Material provided: Live adults
Unit definition: Batch of 200 live adults
Description : Laboratory colony maintained under standard conditions at 26±1 °C, 90% relative humidity and 12/12 light/dark photoperiod. [...]

Phlebotomus papatasi live adults

Place of origin: Turkey, before 2005
Material provided: Live adults
Unit definition: Batch of 200 live adults
Description : Laboratory colony maintained under standard conditions at 26±1 °C, 90% relative humidity and 12/12 light/dark photoperiod. [...]

Phlebotomus orientalis live adults

Place of origin: Ethiopia, 2010
Material provided: Live adults
Unit definition: Batch of 200 live adults
Description : Laboratory colony maintained under standard conditions at 26±1 °C, 90% relative humidity and 12/12 light/dark photoperiod. [...]

Phlebotomus duboscqi live adults

Place of origin: Senegal, before 1994
Material provided: Live adults
Unit definition: Batch of 200 live adults
Description : Laboratory colony maintained under standard conditions at 26±1 °C, 90% relative humidity and 12/12 light/dark photoperiod. [...]

Phlebotomus argentipes live adults

Place of origin: India, 2008
Material provided: Live adults
Unit definition: Batch of 200 live adults
Description : Laboratory colony maintained under standard conditions at 26±1 °C, 90% relative humidity and 12/12 light/dark photoperiod. [...]

Phlebotomus tobbi infected with Leishmania species

Place of origin: Turkey, 2008
Material provided: Fixed sample or extract
Unit definition: 100 infected specimens
Description : 3-5 day old females are fed through chick-skin membrane on the mixture of heat inactivated rabbit blood and Leishmania promastigotes [...]

Phlebotomus sergenti infected with Leishmania species

Place of origin: Spain, 2005
Material provided: Fixed sample or extract
Unit definition: 100 infected specimens
Description : 3-5 day old females are fed through chick-skin membrane on the mixture of heat inactivated rabbit blood and Leishmania promastigotes [...]

Phlebotomus papatasi infected with Leishmania species

Place of origin: Turkey, before 2005
Material provided: Fixed sample or extract
Unit definition: 100 infected specimens
Description : 3-5 day old females are fed through chick-skin membrane on the mixture of heat inactivated rabbit blood and Leishmania promastigotes [...]

Phlebotomus perniciosus infected with Leishmania species

Place of origin: Spain, before 1994
Material provided: Fixed sample or extract
Unit definition: 100 infected specimens
Description : 3-5 day old females are fed through chick-skin membrane on the mixture of heat inactivated rabbit blood and Leishmania promastigotes [...]

Phlebotomus orientalis infected with Leishmania species

Place of origin: Ethiopia, before 2010
Material provided: Fixed sample or extract
Unit definition: 100 infected specimens
Description : 3-5 day old females are fed through chick-skin membrane on the mixture of heat inactivated rabbit blood and Leishmania promastigotes [...]

High-throughput screen of live insects

Material provided: Screening results provided electronically
Unit definition: 500 specimens tested
Description : Up to 500 test substances can be tested against Anopheles stephensi mosquitoes or Plasmodium falciparum infected A. stephensi.

Phlebotomus argentipes infected with Leishmania species

Place of origin: India, before 2008
Material provided: Fixed sample or extract
Unit definition: 100 infected specimens
Description : 3-5 day old females are fed through chick-skin membrane on the mixture of heat inactivated rabbit blood and Leishmania promastigotes [...]

Phlebotomus arabicus infected with Leishmania species

Place of origin: Brazil, 2014
Material provided: Fixed sample or extract
Unit definition: 100 infected specimens
Description : 3-5 day old females are fed through chick-skin membrane on the mixture of heat inactivated rabbit blood and Leishmania promastigotes [...]

Phlebotomus arabicus live adults

Place of origin: Israel, 2003
Material provided: Live adults
Unit definition: Batch of 200 live adults
Description : Laboratory colony maintained under standard conditions at 26±1 °C, 90% relative humidity and 12/12 light/dark photoperiod. [...]

Lutzomyia migonei infected with Leishmania species

Place of origin: Brazil, 2014
Material provided: Fixed sample or extract
Unit definition: 100 infected specimens
Description : 3-5 day old females are fed through chick-skin membrane on the mixture of heat inactivated rabbit blood and Leishmania promastigotes [...]

Lutzomyia migonei preserved specimens

Place of origin: Brazil, 2014
Material provided: Fixed sample or extract
Unit definition: 100 preserved specimens
Description : Laboratory colony maintained under standard conditions at 26±1 °C, 90% relative humidity and 12/12 light/dark photoperiod. [...]

Lutzomyia migonei live adults

Place of origin: Brazil, 2014
Material provided: Live adults
Unit definition: Batch of 200 live adults
Description : Laboratory colony maintained under standard conditions at 26±1 °C, 90% relative humidity and 12/12 light/dark photoperiod. [...]

Anopheles stephensi strain AF-SDA500 infected with Plasmodium falciparum

Place of origin: Pakistan, 1982
Material provided: Fixed sample or extract
Unit definition: 50 infected females
Description : 1-3 day-old female mosquitoes are fed on an infectious blood-meal using a membrane feeding device […]

Anopheles stephensi strain AF-SDA500 preserved specimens

Place of origin: Pakistan, 1982
Material provided: Fixed sample or extract
Unit definition: 50 preserved specimens
Description : Mosquitoes were reared according to standard procedures.

Anopheles stephensi strain AF-SDA500 live eggs

Place of origin: Pakistan, 1982
Material provided: Live eggs
Unit definition: Batch of >5000 live eggs
Description : Mosquitoes were reared according to standard procedures.

Anopheles darlingi strain Saint George preserved specimens

Place of origin: Saint Georges, French Guiana, 2016
Material provided: Fixed sample or extract
Unit definition: 50 preserved mosquitoes
Description : Availability from 2018.

Anopheles darlingi strain Saint George live eggs

Place of origin: Saint Georges, French Guiana, 2016
Material provided: Live eggs
Unit definition: Batch of >1000 live eggs
Description : Availability from 2018.

Culicoides sonorensis preserved specimens

Material provided: Fixed sample or extract
Unit definition: 200 preserved specimens
Description : Information coming soon

Culicoides nubeculosus preserved specimens

Material provided: Fixed sample or extract
Unit definition: 200 preserved specimens
Description : Information coming soon

Culicoides sonorensis live eggs

Material provided: Live eggs
Unit definition: 200 live eggs
Description : Information coming soon

Culicoides nubeculosus live eggs

Material provided: Live eggs
Unit definition: 200 live eggs
Description : Information coming soon

Glossina tachinoides viable pupae

Place of origin: Burkina Faso, 1965
Material provided: Viable pupae
Unit definition: 200 pupae
Description : Production by batch of 50 pupae (same date), sending by 200 (4 batches of 50) [...]

Glossina palpalis viable pupae

Place of origin: Kaduna, Nigeria, 1981
Material provided: Viable pupae
Unit definition: 200 pupae
Description : Production by batch of 50 pupae (same date), sending by 200 (4 batches of 50) [...]

Glossina palpalis gambiensis viable pupae

Place of origin: Burkina Faso, 1968
Material provided: Viable pupae
Unit definition: 200 pupae
Description : Production by batch of 50 pupae (same date), sending by 200 (4 batches of 50) [...]

Glossina tachinoides preserved specimens

Place of origin: Burkina Faso, 1965
Material provided: Fixed sample or extract
Unit definition: 200 preserved specimens
Description : Information coming soon.

Glossina palpalis preserved specimens

Place of origin: Kaduna, Nigeria, 1981
Material provided: Fixed sample or extract
Unit definition: 200 preserved specimens
Description : Information coming soon.

Glossina palpalis gambiensis preserved specimens

Place of origin: Burkina Faso, 1968
Material provided: Fixed sample or extract
Unit definition: 200 preserved specimens
Description : Information coming soon.

Glossina morsitans morsitans preserved specimens

Material provided: Fixed sample or extract
Unit definition: 200 preserved specimens
Description : Information coming soon.

Glossina morsitans morsitans viable pupae

Material provided: Viable pupae
Unit definition: 200 pupae
Description : Production by batch of 50 pupae (same date), sending by 200 (4 batches of 50) [...]

Glossina fuscipes fuscipes preserved specimens

Place of origin: Central African Republic, 1968
Material provided: Fixed sample or extract
Unit definition: 200 preserved specimens
Description : Information coming soon.

Glossina fuscipes fuscipes viable pupae

Place of origin: Central African Republic, 1968
Material provided: Viable pupae
Unit definition: 200 pupae
Description : Production by batch of 50 pupae (same date), sending by 200 (4 batches of 50) [...]

Lutzomyia longipalpis preserved specimens

Place of origin: Brazil, before 1991
Material provided: Fixed sample or extract
Unit definition: 100 preserved specimens
Description : Laboratory colony maintained under standard conditions at 26±1 °C, 90% relative humidity [...]

Lutzomyia longipalpis infected with Leishmania species

Place of origin: Brazil, before 1991
Material provided: Fixed sample or extract
Unit definition: 100 infected specimens
Description : 3-5 day old females are fed through chick-skin membrane on the mixture of heat inactivated rabbit blood and Leishmania promastigotes [...]

Ornithodoros porcinus preserved specimens

Place of origin: Mahitsy, Madagascar, 2012
Material provided: Fixed sample or extract
Unit definition: Batch of 8 preserved specimens
Description : Ticks are reared under specific conditions (no light – 28°C – 85% RH) using only artificial blood feeding. […]

Ornithodoros porcinus live adults

Place of origin: Mahitsy, Madagascar, 2012
Material provided: Live adults
Unit definition: Batch of 8 live adults
Description : Ticks are reared under specific conditions (no light – 28°C – 85% RH) using only artificial blood feeding. […]

Ornithodoros moubata preserved specimens

Place of origin: Neuchâtel University, 2008
Material provided: Fixed sample or extract
Unit definition: Batch of 8 preserved specimens
Description : Ticks are reared under specific conditions (no light – 28°C – 85% RH) using only artificial blood feeding. […]

Ornithodoros moubata live adults

Place of origin: Neuchâtel University, 2008
Material provided: Live adults
Unit definition: Batch of 8 live adults
Description : Ticks are reared under specific conditions (no light – 28°C – 85% RH) using only artificial blood feeding. […]

Ornithodoros erraticus preserved specimens

Place of origin: Alentejo, Portugal, 2013
Material provided: Fixed sample or extract
Unit definition: Batch of 8 preserved specimens
Description : Ticks are reared under specific conditions (no light – 28°C – 85% RH) using only artificial blood feeding. […]

Ornithodoros erraticus live adults

Place of origin: Alentejo, Portugal, 2013
Material provided: Live adults
Unit definition: Batch of 8 live adults
Description : Ticks are reared under specific conditions (no light – 28°C – 85% RH) using only artificial blood feeding. […]

Stomoxys calcitrans preserved specimens

Material provided: Fixed sample or extract
Unit definition: 200 preserved specimens
Description : Rearing rooms maintained at 26°C ± 1°C and 16:8 light: dark cycle. Adults are fed using defibrinated horse blood on cotton wool in a disposable weigh boat. [...]

Stomoxys calcitrans viable pupae

Material provided: Viable pupae
Unit definition: 200 pupae
Description : Rearing rooms maintained at 26°C ± 1°C and 16:8 light: dark cycle. Adults are fed using defibrinated horse blood on cotton wool in a disposable weigh boat. [...]

Aedes aegypti wild-collected preserved specimens, New Caledonia

Place of origin: New Caledonia
Material provided: Fixed sample or extract
Unit definition: 100 preserved specimens
Description : Wild larvae or adult mosquitoes will be collected in New Caledonia.

Aedes aegypti wild-collected live eggs, New Caledonia

Place of origin: New Caledonia
Material provided: Live eggs from wild-collected samples
Unit definition: 3 egg layings (about 10,000 to 30,000 eggs)
Description : Wild samples will be collected in New Caledonia to produce eggs of this vector species.

Anopheles coluzzii strain G3 MRA-112, mutagenesis transgenic line

Place of origin: McCarthy Island, the Gambia, 1975
Material provided: Transgenic line
Unit definition: 1 mosquito transgenic line (batch of >2500 eggs)
Description : Mosquito transgenic line created by CRISPR–Cas9 mediated mutagenesis.

Anopheles coluzzii strain G3 MRA-112, PiggyBac transgenic line

Place of origin: McCarthy Island, the Gambia, 1975
Material provided: Transgenic line
Unit definition: 1 mosquito transgenic line (batch of >2500 eggs)
Description : Mosquito transgenic line created by PiggyBac.

Anopheles coluzzii strain N’Gousso preserved specimens

Place of origin: Yaoundé, Cameroon, 2006
Material provided: Fixed sample or extract
Unit definition: 50 preserved specimens
Description : Mosquitoes were reared according to standard procedures.

Anopheles coluzzii strain N’Gousso live eggs

Place of origin: Yaoundé, Cameroon, 2006
Material provided: Live eggs
Unit definition: Batch of >5000 live eggs
Description : Mosquitoes were reared according to standard procedures.

Anopheles coluzzii strain N’Gousso infected with Plasmodium falciparum

Place of origin: Yaoundé, Cameroon, 2006
Material provided: Fixed sample or extract
Unit definition: 50 infected females
Description : 1-3 day-old female mosquitoes are fed on an infectious blood-meal using a membrane feeding device. Unfed females are removed and fully engorged females are maintained under standard conditions on a 10% sucrose solution.

Anopheles coluzzii strain N’Gousso infected with Plasmodium falciparum

Place of origin: Yaoundé, Cameroon, 2006
Material provided: Fixed sample or extract
Unit definition: 50 infected females
Description : A. coluzzii Ngousso mosquitoes are fed through membrane feeders at 37°C for 15 min on the NF54 gametocyte cultures with a final gametocytaemia ranging between 0.15 to 0.30%. […]

Anopheles coluzzii strain N’Gousso, with custom gene silencing, infected with Plasmodium falciparum

Place of origin: Yaoundé, Cameroon, 2006
Material provided: Fixed sample or extract
Unit definition: 50 gene-silenced infected females
Description : Specific dsRNA will be provided by the end-user. A. coluzzii Ngousso mosquitoes are microinjected with specific dsRNA at requested time before infection.

Culex pipiens strain Caldbeck preserved specimens

Place of origin: Caldbeck (Surrey, UK), 2011
Material provided: Fixed sample or extract
Unit definition: 50 preserved mosquitoes
Description : Rearing rooms maintained at 25°C ± 1°C and 16:8 light:dark cycle. For colony maintenance adults are given defibrinated horse blood via a Hemotek membrane feeder. […]

Culex pipiens strain Caldbeck live adults

Place of origin: Caldbeck (Surrey, UK), 2011
Material provided: Live adults
Unit definition: Batch of 200 adults, mixed-sex
Description : Rearing rooms maintained at 25°C ± 1°C and 16:8 light:dark cycle. For colony maintenance adults are given defibrinated horse blood via a Hemotek membrane feeder. […]

Culex pipiens strain Gavà, infected with West Nile virus strain NY 389-99

Place of origin: Gavà, Catalonia, Spain, 2012
Material provided: Fixed sample or extract
Unit definition: 50 infected females
Description : 10-12 days old females are fed on an infectious blood-meal using the Hemotek® system or intra-thoracically inoculated. […]

Culex pipiens strain Gavà, infected with West Nile virus strain PaAn001

Place of origin: Gavà, Catalonia, Spain, 2012
Material provided: Fixed sample or extract
Unit definition: 50 infected females
Description : 10-12 days old females are fed on an infectious blood-meal using the Hemotek® system or intra-thoracically inoculated. […]

Culex pipiens strain Brookwood preserved specimens

Place of origin: Brookwood (Surrey, UK), 2011
Material provided: Fixed sample or extract
Unit definition: 50 preserved mosquitoes
Description : Rearing rooms maintained at 25°C ± 1°C and 16:8 light:dark cycle. For colony maintenance adults are given defibrinated horse blood via a Hemotek membrane feeder. […]

Culex pipiens strain Brookwood live adults

Place of origin: Brookwood (Surrey, UK), 2011
Material provided: Live adults
Unit definition: Batch of 200 adults, mixed-sex
Description : Rearing rooms maintained at 25°C ± 1°C and 16:8 light:dark cycle. For colony maintenance adults are given defibrinated horse blood via a Hemotek membrane feeder. […]

Aedes albopictus strain Rimini live eggs

Place of origin: Rimini, Italy, 2004
Material provided: Live eggs
Unit definition: Batch of >5000 live eggs
Description : Rearing rooms maintained at 25°C ± 1°C and 16:8 light:dark cycle. […]

Aedes albopictus strain Mauritius-2013 live eggs

Place of origin: Mauritius, 2013
Material provided: Live eggs
Unit definition: Batch of >5000 live eggs
Description : Rearing rooms maintained at 25°C ± 1°C and 16:8 light:dark cycle […]

Vector experimental infections with CL2 and CL3 arboviruses

Location: Centre de Recerca en Sanitat Animal (CReSA), Barcelona, Spain
Material provided: Physical access for end-users
Unit definition: 1 week of access
Biosafety level: CL2/CL3
Description : Equipped CL2 and CL3 lab to undertake experimental infections on Aedes albopictus and Culex pipiens with arboviruses: Dengue, Chikungunya, Zika, West Nile and Rift Valley fever virus.

Arthropod vector experimental infections with viruses or parasites

Location: Centre de coopération Internationale en Recherche Agronomique pour le Déveoppement (CIRAD), Montpellier, France
Material provided: Physical access for end-users
Unit definition: 1 week of access
Biosafety level: CL2
Description : Access to a polyvalent insectarium for experimental infections with arthropods with CL2 pathogens.

Mosquito behaviour experiments in large field cages

Location: Centro Agricoltura Ambiente “G.Nicoli” (CAA), Crevalcore, Italy
Material provided: Physical access for end-users
Unit definition: 1 week of access
Biosafety level: CL1
Description : Four large cages (8 x 5 x 2.8 meters or smaller) in wooded area with irrigation system can host behaviour experiments from May to September.

Mosquito experimental infections with Plasmodium falciparum

Location: Max Planck Institute for Infection Biology (MPIIB), Berlin, Germany
Material provided: Physical access for end-users
Unit definition: 1 week of access
Biosafety level: CL2
Description : Fully equipped CL2 lab to undertake experimental infections of Anopheles with Plasmodium falciparum.

Vector behaviour experiments with arboviruses

Location: Wageningen University (WU), Wageningen, Netherlands
Material provided: Physical access for end-users
Unit definition: 1 week of access
Biosafety level: CL3
Description : Fully equipped CL3 lab to study behaviour of vectors infected with Flaviviruses, Alphaviruses and Bunyaviruses.

Vector experimental infections with arboviruses

Location: Wageningen University (WU), Wageningen, Netherlands
Material provided: Physical access for end-users
Unit definition: 1 week of access
Biosafety level: CL3
Description : Fully equipped CL3 lab to undertake vector infections with Flaviviruses, Alphaviruses and Bunyaviruses

Phlebotomus arabicus preserved specimens

Place of origin: Israel, 2003
Material provided: Fixed sample or extract
Unit definition: 100 preserved specimens
Description : Laboratory colony maintained under standard conditions at 26±1 °C, 90% relative humidity and 12/12 light/dark photoperiod. […]

Phlebotomus argentipes preserved specimens

Place of origin: India, before 2008
Material provided: Fixed sample or extract
Unit definition: 100 preserved specimens
Description : Laboratory colony maintained under standard conditions at 26±1 °C, 90% relative humidity

Phlebotomus duboscqi adult preserved specimens

Place of origin: Senegal, before 1994
Unit definition: Batch of 200 adults
Biosafety level: Psychodidae, Phlebotomus duboscqi
Description : Laboratory colony maintained under standard conditions at 26±1 °C, 90% relative humidity.

Aedes aegypti strain AALVP with custom gene silencing

Place of origin: West Africa, 1936
Material provided: Fixed sample or extract
Unit definition: 50 gene-silenced mosquitoes
Description : One unit is 50 mosquitoes injected with 2µg of dsRNA of interest plus 50 mosquitoes injected with dslacZ (control) plus rearing and sampling [...]

Aedes aegypti strain LVP infected with Zika virus

Place of origin: West Africa
Material provided: Inactivated sample
Unit definition: 50 infected females
Description : 1-week-old females are fed on an infectious blood-meal using the Hemotek® system.

Aedes aegypti strain LVP infected with dengue virus

Place of origin: West Africa
Biosafety level: Culicidae; Aedes aegypti
Description : 1-week-old females are fed on an infectious blood-meal using the Hemotek® system.

Aedes aegypti strain AALVP infected with chikungunya virus

Place of origin: West Africa
Unit definition: 50 infected females
Biosafety level: Culicidae; Aedes aegypti
Description : 1-week-old females are fed on an infectious blood-meal using the Hemotek® system.

Aedes aegypti strain LVP preserved specimens

Place of origin: West Africa
Biosafety level: Culicidae; Aedes aegypti
Description : Eggs are hatched in dechlorinated tap water and larvae are reared under controlled conditions.

Aedes aegypti strain AALVP live eggs

Place of origin: West Africa
Material provided: Live eggs
Unit definition: Batch of >5000 live eggs
Description : Eggs are hatched in dechlorinated tap water and larvae are reared under controlled conditions. [...]

Aedes aegypti strain AAPAEA with custom gene silencing, infected with Zika virus

Place of origin: Paea, Tahiti (French Polynesia), 1994
Material provided: Fixed sample or extract
Unit definition: 50 gene-silenced infected females
Description : One unit is 50 mosquitoes injected with 2µg of dsRNA of interest plus 50 mosquitoes injected with dslacZ (control) plus rearing and sampling […]

Aedes aegypti strain AAPAEA with custom gene silencing, infected with dengue virus

Place of origin: Paea Tahiti (French Polynesia), 1994
Material provided: Fixed sample or extract
Unit definition: 50 gene-silenced infected females
Description : One unit is 50 mosquitoes injected with 2µg of dsRNA of interest plus 50 mosquitoes injected with dslacZ (control) plus rearing and sampling [...]

Phlebotomus orientalis adults preserved specimens

Place of origin: Ethiopia, before 2010
Biosafety level: Psychodidae, Phlebotomus orientalis
Description : Laboratory colony maintained under standard conditions at 26±1 °C, 90% relative humidity.

Phlebotomus papatasi preserved specimens

Place of origin: Turkey, before 2005
Material provided: Fixed sample or extract
Unit definition: 100 preserved specimens
Description : Laboratory colony maintained under standard conditions at 26±1 °C, 90% relative humidity and 12/12 light/dark photoperiod. […]

Phlebotomus perniciosus strain Murcia adults preserved specimens

Place of origin: Spain, before 1994
Biosafety level: Psychodidae, Phlebotomus perniciosus
Description : Laboratory colony maintained under standard conditions at 26±1 °C, 90% relative humidity

Phlebotomus sergenti preserved specimens

Place of origin: Turkey, before 2005
Material provided: Fixed sample or extract
Unit definition: 100 preserved specimens
Description : Laboratory colony maintained under standard conditions at 26±1 °C, 90% relative humidity and 12/12 light/dark photoperiod. [...]

Phlebotomus tobbi adults preserved specimens

Place of origin: Turkey, 2008
Biosafety level: Psychodidae, Phlebotomus tobbi
Description : Laboratory colony maintained under standard conditions at 26±1 °C, 90% relative humidity

Aedes aegypti strain AAPAEA with custom gene silencing

Place of origin: Paea, Tahiti (French Polynesia), 1994
Material provided: Fixed sample or extract
Unit definition: 50 gene-silenced mosquitoes
Description : One unit is 50 mosquitoes injected with 2µg of dsRNA of interest plus 50 mosquitoes injected with dslacZ (control) plus rearing and sampling [...]

Sergentomyia schwetzi A adults preserved specimens

Place of origin: Ethiopia, 2010
Biosafety level: Psychodidae, Sergentomyia schwetzi A
Description : Laboratory colony maintained under standard conditions at 26±1 °C, 90% relative humidity

Aedes aegypti strain AAPAEA with custom gene silencing, infected with Chikungunya virus

Place of origin: Paea Tahiti (French Polynesia), 1994
Material provided: Fixed sample or extract
Unit definition: 50 gene-silenced females
Description : One unit is 50 mosquitoes injected with 2µg of dsRNA of interest plus 50 mosquitoes injected with dslacZ (control) plus rearing and sampling […]

Sergentomyia schwetzi B adults preserved specimens

Place of origin: Ethiopia, 2010
Biosafety level: Psychodidae; Sergentomyia schwetzi B
Description : Laboratory colony maintained under standard conditions at 26±1 °C, 90% relative humidity.

Lutzomyia longipalpis live adults

Place of origin: Brazil, before 1991
Material provided: Live adults
Unit definition: Batch of 200 live adults
Description : Laboratory colony maintained under standard conditions at 26±1 °C, 90% relative humidity and 12/12 light/dark photoperiod. [...]

Mosquito behaviour experiments in CL2 large cages

Location: Polo d’Innovazione di Genomica, Genetica e Biologia (PoloGGB), Perugia, Italy
Material provided: Physical access for end-users
Unit definition: 1 week of access
Biosafety level: CL2
Description : Large cage experiments under near-natural conditions in environmental chambers at biosafety level CL2.

RNAseq total transcriptome Illumina sequencing

Material provided: Data (raw sequence files); return of left-over RNA sample
Unit definition: 12 samples/lane (4 experimental conditions @ 3 replicates/condition)
Description : Total RNA-Seq analyzes both coding and multiple forms of noncoding RNA for a comprehensive view of the transcriptome.

Bioinformatic analysis for RNAseq total transcriptome Illumina sequencing

Material provided: Data
Description : Bioinformatics enables to analyse similar datasets from quality control, genome mapping (if a reference genome is available), transcriptome assembly, differential gene expression, statistical testing and downstream analysis (GO terms assignment and pathway analysis).

Aedes albopictus strain ALPROV live eggs

Place of origin: La Providence, Saint-Denis, La Réunion, 2010
Biosafety level: Culicidae; Aedes albopictus.
Description : Eggs are hatched in dechlorinated tap water and larvae are reared under controlled conditions.

Aedes albopictus strain ALPROV preserved specimens

Place of origin: La Providence, Saint-Denis, La Réunion, 2010
Biosafety level: Culicidae; Aedes albopictus.
Description : Eggs are hatched in dechlorinated tap water and larvae are reared under controlled conditions.

Aedes albopictus strain ALPROV infected with dengue virus

Place of origin: La Providence, Saint-Denis, La Réunion, 2010
Biosafety level: Culicidae; Aedes albopictus.
Description : 1-week-old females are fed on an infectious blood-meal using the Hemotek® system.

Aedes albopictus strain ALPROV infected with Zika virus

Place of origin: La Providence, Saint-Denis, La Réunion, 2010
Material provided: Fixed whole or partial mosquito samples
Unit definition: Non-infectious material derived from 50 infected females
Description : 1-week-old females are fed on an infectious blood-meal using the Hemotek® system.

Aedes albopictus strain ALPROV infected with Chikungunya virus

Place of origin: La Providence, Saint-Denis, La Réunion, 2010
Material provided: Fixed whole or partial mosquito samples
Unit definition: Non-infectious material derived from 50 infected females
Description : 1-week-old females are fed on an infectious blood-meal using the Hemotek® system.

Aedes albopictus strain ALNICE preserved specimens

Place of origin: Nice, France, 2011
Biosafety level: Culicidae; Aedes albopictus.
Description : Eggs are hatched in dechlorinated tap water and larvae are reared under controlled conditions

Aedes albopictus strain ALNICE live eggs

Place of origin: Nice, France, 2011
Biosafety level: Culicidae; Aedes albopictus.
Description : Eggs are hatched in dechlorinated tap water and larvae are reared under controlled conditions.

Aedes albopictus strain ALNICE Infected with Zika virus

Place of origin: Nice, France, 2011
Biosafety level: Culicidae; Aedes albopictus.
Description : 1-week-old females are fed on an infectious blood-meal using the Hemotek® system.

Aedes albopictus strain ALNICE Infected with dengue virus

Place of origin: Nice, France, 2011
Unit definition: 50 infected females
Biosafety level: Culicidae; Aedes albopictus
Description : 1-week-old females are fed on an infectious blood-meal using the Hemotek® system.

Aedes albopictus strain ALNICE Infected with Chikungunya virus

Place of origin: Nice, France, 2011
Unit definition: 3
Biosafety level: Culicidae; Aedes albopictus.
Description : 1-week-old females are fed on an infectious blood-meal using the Hemotek® system.

Mosquito experimental infections with CL2 arboviruses

Location: Institut Pasteur de Nouvelle Calédonie (IPNC), Noumea, New Caledonia
Material provided: Physical access for end-users
Unit definition: 1 week of access
Biosafety level: CL2
Description : Equipped CL2 lab to undertake experimental infections on Aedes aegypti from New Caledonia with arboviruses: dengue, chikungunya or Zika.

Vector behaviour experiments with arboviruses

Location: Institut de Recherche pour le Développement (IRD), Montpellier, France
Material provided: Physical access for end-users
Unit definition: 1 week of access
Biosafety level: CL2/CL3
Description : Fully equipped CL3 lab to study behaviour of Aedes colonies infected with arboviruses (dengue, Zika or chikungunya).

Vector experimental infections with arboviruses

Location: Institut de Recherche pour le Développement (IRD), Montpellier, France
Material provided: Physical access for end-users
Unit definition: 1 week of access
Biosafety level: CL2/CL3
Description : Fully equipped CL3 lab to undertake pathogen infections (chikungunya, dengue, Zika) on Aedes colonies.

Mosquito behaviour and insecticide experiments in small cages

Location: Institut de Recherche pour le Développement (IRD), Montpellier, France
Material provided: Physical access for end-users
Unit definition: 1 week of access
Biosafety level: CL1
Description : Fully equipped CL1 lab to study mosquito behaviour using standard and custom devices and protocols, including standard WHO insecticide susceptibility assays.

siRNA cell line screen with CL2 pathogens

Location: UK
Description : Activity screen of Aag2 cells with 1000 siRNAs targeting Ae. aegypti genes, upon exposure to a CL2 pathogen.

Aedes aegypti strain AAPAEA Infected with Chikungunya virus

Place of origin: Paea Tahiti (French Polynesia), 1994
Biosafety level: Culicidae; Aedes aegypti
Description : 1-week-old females are fed on an infectious blood-meal using the Hemotek® system.

Aedes aegypti strain AAPAEA preserved specimens

Place of origin: Paea, Tahiti (French Polynesia), 1994
Biosafety level: Culicidae; Aedes aegypti
Description : Eggs are hatched in dechlorinated tap water and larvae are reared under controlled conditions.

Mosquito mass rearing (up to 100,000 adults)

Location: Italy
Description : Fully equipped CL3 lab to mass rear any mosquito species, including GM strains, using internal SOP or following specific requests from the user.

Mosquito mass rearing (up to 1000 adults)

Location: Italy
Description : Fully equipped CL3 lab to mass rear any mosquito species, including GM strains, using internal SOP or following specific requests from the user.

siRNA cell line screen with CL3 pathogens

Location: UK
Description : Activity screen of Aag2 cells with 1000 siRNAs targeting Ae. aegypti genes, upon exposure to a CL3 pathogen.

Mosquito mass rearing (up to 10,000 adults)

Location: Italy
Description : Fully equipped CL3 lab to mass rear any mosquito species, including GM strains, using internal SOP or following specific requests from the user.

Aedes aegypti strain AAPAEA Infected with Zika virus

Place of origin: Paea, Tahiti (French Polynesia), 1994
Biosafety level: Culicidae; Aedes aegypti
Description : 1-week-old females are fed on an infectious blood-meal using the Hemotek® system.

Aedes aegypti strain AAPAEA Infected with dengue virus

Place of origin: Paea, Tahiti (French Polynesia), 1994
Biosafety level: Culicidae; Aedes aegypti
Description : 1-week-old females are fed on an infectious blood-meal using the Hemotek® system.

Aedes aegypti strain AAPAEA live eggs

Place of origin: Paea Tahiti (French Polynesia), 1994
Material provided: Live eggs
Unit definition: Batch of >5000 live eggs
Description : Eggs are hatched in dechlorinated tap water and larvae are reared under controlled condition [...]

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