Anopheles stephensi strain Sind-Kasur Nijmegen infected with Plasmodium falciparum GFP-Luciferase (HSP promoter)

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Reference: V.2.3.2.I1-1.NL.151.51
Origin: Pakistan, 1982
Material provided: Whole insects, dissected tissues, or extracts
Unit definition: 50 infected females (25 if dissected)
Provider country: The Netherlands (TropIQ Health Sciences B.V.)

A P. falciparum NF54-CGL stage V gametocytes culture with 5×105 exflagellation events / ml is used for feeding of female Anopheles stephensi

SKU: V.2.3.2.I1-1.NL.151.51 Category: Tag:

Description

Material provided: Whole mosquitoes, dissected tissues, or extracts
Unit definition: 50 infected females (25 if dissected) supplied as 100 fully-engorged females with infection prevalence at least 50%

Vector information

Family: Culicidae
Genus: Anopheles
Species: Anopheles stephensi
Strain Name: Sind-Kasur Nijmegen*
Place of origin: Pakistan
Date of colonisation: 1982
*The Kasur strain of A. stephensi was field-collected in 1981 from a cattle shed, in the Punjab province of Pakistan. The susceptible line Sda-500 (Sind) was genetically selected for increased susceptibility (for P. falciparum) from the Sind strain, a mixture of stocks from the Sind Province of Pakistan. These two strains were crossed in 1985.

Parasite information

Phylum: Apicomplexa
Genus: Plasmodium
Species: Plasmodium falciparum
Strain: NF54-ΔPf47-5’hsp70-GFP-Luc (Short: NF54-HGL) expressing a GFP-Luciferase fusion protein under the control of the Pfhsp70 promoter, genomic integration, no selection marker
Infectivity: Infectivity tested and quantified
Mycoplasmic content: Mycoplasma free

Production conditions

A P. falciparum NF54-CGL stage V gametocytes culture with 5×105 exflagellation events / ml is used for feeding of female Anopheles stephensi mosquitoes. On day 8 after infection, infection rate will be determined via luciferase assay.

Product options

Please specify in your request:

Time post-infection: Please specify one time point post-infection to harvest the samples. The most common options are ookinete infection (18-24 h post-infection), oocyst infection (2-13 d post-infection) and sporozoite infection (14-15 d post-infection), but other times are possible by request, contact us.

Whole or dissected: Instead of 50 whole mosquitoes, material can be supplied from dissected material harvested at the appropriate time point: 25 dissected midguts or 25 dissected salivary glands or 25 dissected ovaries, with the corresponding carcasses.

Form of material:

  • Whole mosquitoes or dissected tissues, fixed in 70% ethanol, formalin, or other fixative: useful for DNA extraction from 70% ethanol, immunohistochemistry, not useful for RNA experiments or proteomics. Shipped as non-infectious material.
  • Whole mosquitoes or dissected tissues, homogenised in a lysis buffer such as Trizol: useful for RNA, DNA and protein experiments. Shipped as non-infectious material.
  • Whole mosquitoes or dissected tissues, frozen at -80°C or stored in RNALater: useful for RNA, DNA and protein experiments. Shipped as non-infectious material.
  • Other formats or preparations may be possible by request, contact us.

Production conditions: Material is generated using default gametocyte dose and incubation temperature used by the supplying facility. Other conditions may be possible upon request.

Sample size and replicates: For larger sample sizes, request more units. Please specify if independent infections are needed as biological replicates (e.g., for RNAseq transcriptional profiling experiments).

Publications

  • Feldmann AM, Ponnudurai T 1989, Selection of Anopheles stephensi for refractoriness and susceptibility to Plasmodium falciparum. Med Vet Entomol. 3 (1): 41-52
  • Ponnudurai, T., Leeuwenberg, A. D. and Meuwissen, J. H. (1981) Chloroquine sensitivity of isolates of Plasmodium falciparum adapted to in vitro culture. Tropical and Geographical Medicine 33, 50-54.
  • Vos et al., 2015. A semi-automated luminescence based standard membrane feeding assay identifies novel small molecules that inhibit transmission of malaria parasites by mosquitoes. Sci Rep 5; 18704

For more information, please contact us.

Additional information

Provider country

The Netherlands

Provider(s)

TropIQ Health Sciences B.V. (TropIQ)

Eligibility

In order to conform to H2020 rules promoting scientific interaction between countries, if your institute is located in the provider country, you need to choose another similar product, or form a user group to request this product, Please check: http://infravec2.eu/user-groups/