Phlebotomus orientalis preserved or extracts

0.00

Reference: V.6.4.P.CZ.138.20
Origin: Ethiopia, before 2010
Material provided: Whole sandflies, dissected tissues, or extracts
Unit definition: 200 sandflies (100 if dissected)
Provider country: Czech Republic (Charles University - CUNI)

Laboratory colony maintained under standard conditions at 26±1 °C, 90% relative humidity.

SKU: V.6.4.P.CZ.138.20 Category: Tags: , ,

Description

Material provided: Whole sandflies, dissected tissues, or extracts
Unit definition: 200 sandflies (100 if dissected)
Availability: This product is designed for projects that require up to 10 units of sandflies, infected sandflies or sandfly-derived products (all species combined) per order. The product is particularly aimed at pilot and exploratory-scale projects. Please contact the TNA Manager for larger requests or additional information.

Vector information

Family: Psychodidae
Genus: Phlebotomus
Species: Phlebotomus orientalis
Place of origin: Ethiopia
Date of colonisation: before 2010

Production protocol

Laboratory colony maintained under standard conditions at 26±1 °C, 90% relative humidity and 12/12 light/dark photoperiod. Larvae provided with standardized fermented diet (rabbit pellets/rabbit faeces), adults with constant access to cotton wool soaked with 50% sugar and a water source. For more details see the reference below.

Product options

Please specify in your request:

Sample size: For larger sample sizes, request more units.

Whole or dissected: Instead of 200 whole sandflies, material can be supplied from dissected material harvested at the appropriate time point: 100 dissected midguts or 100 dissected salivary glands, provided with the corresponding carcasses.

Form of material:

  • Whole sandflies or dissected tissues, fixed in 70% ethanol, formalin, or other fixative: useful for DNA extraction from 70% ethanol, immunohistochemistry, not useful for RNA experiments or proteomics.
  • Whole sanflies or dissected tissues, homogenised in a lysis buffer such as Trizol: useful for RNA, DNA and protein experiments.
  • Whole sandflies or dissected tissues, frozen at -80°C or stored in RNALater: useful for RNA, DNA and protein experiments.
  • Other formats or preparations may be possible by request, contact us.

Publications

  • Volf et Volfova (2011) Establishment and maintenance of sand fly colonies. J Vector Ecol 36 (Suppl 1): S1-S9.
  • Seblova et al. (2013) Phlebotomus orientalis sand flies from two geographically distant ethiopian localities: biology, genetic analyses and ausceptibility to Leishmania donovani. PLOS NEGLECTED TROPICAL DISEASES  7:  Article N. e2187.
  • Sadlova et al. (2015) Xenodiagnosis of Leishmania donovani in BALB/c mice using Phlebotomus orientalis: a new laboratory model. Parasit Vectors. 8:158.

For more information, please contact us.

Additional information

Provider country

Czech Republic

Provider(s)

Charles University (CUNI)

Eligibility

In order to conform to H2020 rules promoting scientific interaction between countries, if your institute is located in the provider country, you need to choose another similar product, or form a user group to request this product, Please check: http://infravec2.eu/user-groups/