Description
Material provided: Whole mosquitoes, dissected tissues, or extracts
Unit definition: 50 infected females (25 if dissected) supplied as 100 fully-engorged females with infection prevalence at least 50%
Vector information
Family: Culicidae
Genus: Aedes
Species: Aedes albopictus
Strain Name: ALMONTPELLIER-Infravec
Place of origin: Montpellier, France
Date of colonisation: 2018
Virus information
Pathogen used for infection: Chikungunya
Reference: 06.21
ICTV Taxonomy: ssRNA+, Alphavirus, Togaviridae
Virus name: Chikungunya virus
Strain: La Réunion
Sequencing: Accession number AM258992
Infectivity: Infectivity tested and quantified
Mycoplasmic content: Mycoplasma free
Production conditions
1-week-old females are fed on an infectious blood-meal using the Hemotek® system. The titer of infectious blood-meals is 107 PFU/mL. After the infectious blood-meal, engorged females are transferred to small containers and fed with 10% sucrose in a chamber maintained at 28°±1°C, a 16h: 8h light: dark cycle and 80% humidity until day 7 post-infection. Products shipped under non-infectious conditions (RNA, DNA).
Product options: the temperature of incubation and day post-infection can be modified by user request.
Shipping conditions: In alcohol 70% or silicagel at Room Temperature.
Publications
- Jupille H, Seixas G, Mousson L, Sousa CA, Failloux AB (2016) Zika Virus, a New Threat for Europe? PLoS Negl Trop Dis. 10(8):e0004901.
- Schuffenecker I, Iteman I, Michault A, et al. (2006) Genome microevolution of Chikungunya viruses causing the Indian Ocean outbreak. PLoS Medicine, 3, e263.
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