Description
Material provided: Whole sandflies, dissected tissues, or extracts
Unit definition: 200 sandflies (100 if dissected)
Availability: This product is designed for projects that require up to 6 units of an infected vector product and is particularly aimed at pilot and exploratory-scale projects. Please contact the TNA Manager for larger requests or additional information.
Vector information
Family: Psychodidae
Genus: Phlebotomus
Species: Phlebotomus sergenti
Place of origin: Turkey
Date of colonisation: before 2005
Production protocol
Laboratory colony maintained under standard conditions at 26±1 °C, 90% relative humidity and 12/12 light/dark photoperiod. Larvae provided with standardized fermented diet (rabbit pellets/rabbit faeces), adults with constant access to cotton wool soaked with 50% sugar and a water source. For more details see the reference below.
Product options
Please specify in your request:
Sample size: For larger sample sizes, request more units.
Whole or dissected: Instead of 200 whole sandflies, material can be supplied from dissected material harvested at the appropriate time point: 100 dissected midguts or 100 dissected salivary glands, provided with the corresponding carcasses.
Form of material:
- Whole sandflies or dissected tissues, fixed in 70% ethanol, formalin, or other fixative: useful for DNA extraction from 70% ethanol, immunohistochemistry, not useful for RNA experiments or proteomics.
- Whole sanflies or dissected tissues, homogenised in a lysis buffer such as Trizol: useful for RNA, DNA and protein experiments.
- Whole sandflies or dissected tissues, frozen at -80°C or stored in RNALater: useful for RNA, DNA and protein experiments.
- Other formats or preparations may be possible by request, contact us.
Publications
- Volf et Volfova (2011) Establishment and maintenance of sand fly colonies. J Vector Ecol 36 (Suppl 1): S1-S9.
- Jancarova et al. (2016) An increase of larval rearing temperature does not affect the susceptibility of Phlebotomus sergenti to Leishmania tropica but effectively eliminates the gregarine Psychodiella sergenti. Parasites and Vectors 9: 553.
For more information, please contact us.