Description
Material provided: Whole mosquitoes, dissected tissues, or extracts
Unit definition: 100 mosquitoes (50 if dissected)
Vector information
Family: Culicidae
Genus: Aedes
Species: Aedes aegypti
Strain Name: Wild population
Place of origin: New Caledonia
Production protocol
Wild larvae or adult mosquitoes will be collected in New Caledonia.
First option (larvae): A minimum of 100 larvae will be collected from different breeding sites within a radius of 100 m. The collection area will be determined in agreement with the user. Larvae will be morphologically identified, and either preserved as larvae or will be raised to adult in the insectary of IPNC, and then preserved.
Second option (adults): Adult collection by BG traps. A minimum of 100 adults will be collected in different traps placed within a radius of 100 m. The collection area will be determined in agreement with the user.
The samples can be stored in 70% ethanol, or dried on silica gel.
Product options
Please specify in your request:
Sample size: For larger sample sizes, request more units.
Whole or dissected: 100 whole mosquitoes; or 50 dissected midguts, salivary glands or ovaries
Form of material:
- Whole mosquitoes or dissected tissues, fixed in 70% ethanol, formalin, or other fixative: useful for DNA extraction from 70% ethanol, immunohistochemistry, not useful for RNA experiments or proteomics.
- Whole mosquitoes or dissected tissues, homogenised in a lysis buffer such as Trizol: useful for RNA, DNA and protein experiments.
- Whole mosquitoes or dissected tissues, frozen at -80°C or stored in RNALater useful for RNA, DNA and protein experiments.
- Other formats or preparations may be possible by request, contact us.
Publications
Calvez, E., L. Guillaumot, L. Millet, J. Marie, H. Bossin, V. Rama, A. Faamoe, S. Kilama, M. Teurlai, F. Mathieu-Daudé and M. Dupon-Rouzeyrol, Genetic diversity and phylogeny of Aedes aegypti, the main arbovirus vector in the Pacific. PLoS Negl Trop Dis, 2016. 10(1): p. e0004374.
For more information, please contact us.
