Description
Material provided: Fixed sample or extracts
Unit definition: 50 infected females
Storage conditions: -80°C
Vector information
Family: Mosquitoes
Genus: Aedes
Species: Aedes albopictus
Strain Name: ALMONTPELLIER-Infravec
Place of origin: Montpellier, France
Date of colonisation: 2018
Virus information
Pathogens used for infection: Dengue
Reference: DENV-2
ICTV Taxonomy: ssRNA+, Flavivirus, Flaviviridae
Virus name: Dengue virus
Strain: Bangkok 1974
Sequencing: Complete genome
Infectivity: Infectivity tested and quantified
Mycoplasmic content: Mycoplasma free
Production protocol
1-week-old females are fed on an infectious blood-meal using the Hemotek® system. The titer of infectious blood-meals is 107 FFU/mL. After the infectious blood-meal, engorged females are transferred to small containers and fed with 10% sucrose in a chamber maintained at 28°±1°C, a 16h: 8h light: dark cycle and 80% humidity until day 14 post-infection. Products shipped under non-infectious conditions (RNA, DNA).
Product options: the temperature of incubation and day post-infection can be modified by user request.
Shipping conditions: Non-infectious. In dry ice.
Publications
- Jupille H, Seixas G, Mousson L, Sousa CA, Failloux AB (2016) Zika Virus, a New Threat for Europe? PLoS Negl Trop Dis. 10(8):e0004901.
- Rosen L, Gubler D (1974) The use of mosquitoes to detect and propagate dengue viruses. Am J Trop Med Hyg 23: 1153–1160.
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