Description
Material provided:Fixed sample or extract
Unit definition: 50 infected females
Storage conditions: -80°C
Vector information
Family: Culicidae
Genus: Aedes
Species: Aedes albopictus
Strain Name: ALMONTPELLIER-Infravec
Place of origin: Montpellier, France
Date of colonisation: 2018
Virus information
Pathogens used for infection: Zika
Reference: PE243
ICTV Taxonomy: ssRNA(+) / Flaviviridae / Flavivirus / Zika virus
Virus name: Zika virus
Strain: Recife/Brazil
Sequencing: Accession number KX197192
Infectivity: Infectivity tested and quantified
Mycoplasmic content: Mycoplasma free
Production protocol
1-week-old females are fed on an infectious blood-meal using the Hemotek® system. The titer of infectious blood-meals is 107 TCID50/mL. After the infectious blood-meal, engorged females are transferred to small containers and fed with 10% sucrose in a chamber maintained at 28°±1°C, a 16h: 8h light: dark cycle and 80% humidity until day 14 post-infection. Products shipped under non-infectious conditions (RNA, DNA).
Product options: the temperature of incubation and day post-infection can be modified by user request.
Shipping conditions: Non-infectious. In dry ice.
Publications
- Jupille H, Seixas G, Mousson L, Sousa CA, Failloux AB (2016) Zika Virus, a New Threat for Europe? PLoS Negl Trop Dis. 10(8):e0004901.
- Donald CL, Brennan B, Cumberworth SL, Rezelj VV, Clark JJ, Cordeiro MT, Freitas de Oliveira França R, Pena L2, Wilkie GS, Da Silva Filipe A, Davis C, Hughes J, Varjak M, Selinger M, Zuvanov L, Owsianka AM, Patel AH, McLauchlan J, Lindenbach BD, Fall G, Sall AA, Biek R, Rehwinkel J, Schnettler E, Kohl A. 2016. Full Genome Sequence and sfRNA Interferon Antagonist Activity of Zika Virus from Recife, Brazil. PLoS Negl Trop Dis 10(10):e0005048.
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