Aedes albopictus strain Vallés preserved or extract

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Reference: V.1.2.7.P.SP.44.8
Origin: Spain, 2005
Material provided: Whole mosquitoes, dissected tissues, or extracts
Unit definition: 100 preserved mosquitoes (50 if dissected)
Provider country: Spain (Institut de Recerca i Tecnologia Agroalimentàries - IRTA)

Eggs are hatched in dechlorinated tap water and larvae are reared under controlled conditions.

SKU: V.1.2.7.P.SP.44.8 Category: Tag:

Description

Material provided: Whole mosquitoes, dissected tissues, or extracts
Unit definition: 100 preserved mosquitoes (50 if dissected)

Vector information

Family: Culicidae
Genus: Aedes
Species: Aedes albopictus
Strain Name: Vallés
Place of origin: Sant Cugat del Vallès, Catalonia (Spain)
Date of colonisation: 2005

Production protocol

Eggs are hatched in dechlorinated tap water and larvae are reared under controlled conditions of 150–200 larvae per 1 liter and fed with one yeast tablet renewed every 3–4 days. Adults are kept in cages at 28°±1°C with a 16h: 8h light: dark cycle, 80% relative humidity, and supplied with a 10% sucrose solution.

Product options

Please specify in your request:

Whole or dissected: 100 whole mosquitoes; or 50 dissected midguts, salivary glands or ovaries

Form of material:

  • Whole mosquitoes or dissected tissues, fixed in 70% ethanol, formalin, or other fixative: useful for DNA extraction from 70% ethanol, immunohistochemistry, not useful for RNA experiments or proteomics.
  • Whole mosquitoes or dissected tissues, homogenised in a lysis buffer such as Trizol: useful for RNA, DNA and protein experiments.
  • Whole mosquitoes or dissected tissues, frozen at -80°C or stored in RNALater useful for RNA, DNA and protein experiments.
  • Other formats or preparations may be possible by request, contact us.

Sample size: For larger sample sizes, request more units.

Publications

  • Brustolin M, Talavera S, Nuñez A, Santamaría C, Rivas R, Pujol N, Valle M, Verdún M, Brun A, Pagès N, Busquets N. Rift Valley fever virus and European mosquitoes: vector competence of Culex pipiens and Stegomyia albopicta (= Aedes albopictus). Med Vet Entomol. 2017 Dec;31(4):365-372. doi: 10.1111/mve.12254. Epub 2017 Aug 7.
  • Brustolin M, Talavera S, Santamaría C, Rivas R, Pujol N, Aranda C, Marquès E, Valle M, Verdún M, Pagès N, Busquets N. Culex pipiens and Stegomyia albopicta (= Aedes albopictus) populations as vectors for lineage 1 and 2 West Nile virus in Europe. Med Vet Entomol. 2016 Jun;30(2):166-73. doi: 10.1111/mve.12164. Epub 2016 Feb 18.

For more information, please contact us.

Additional information

Provider country

Spain

Provider(s)

Institut de Recerca i Tecnologia Agroalimentària (IRTA)

Eligibility

In order to conform to H2020 rules promoting scientific interaction between countries, if your institute is located in the provider country, you need to choose another similar product, or form a user group to request this product, Please check: http://infravec2.eu/user-groups/