Anopheles coluzzii strain N’Gousso preserved or extracts

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Reference: V.2.1.1.P.FR.4.1
Origin: Yaoundé, Cameroon, 2006
Material provided: Whole mosquitoes, dissected tissues, or extracts 
Unit definition: 100 mosquitoes (50 if dissected)
Provider country: France (Institut Pasteur - IP)

Mosquitoes were reared according to standard procedures.

 

SKU: V.2.1.1.P.FR.4.1 Category: Tags: ,

Description

Material provided: Whole mosquitoes, dissected tissues, or extracts
Unit definition: 100 mosquitoes (50 if dissected)

Vector information

Family: Culicidae
Genus: Anopheles 
Species: Anopheles coluzzii
Strain Name: N‘Gousso
Place of origin: Yaoundé, Cameroon
Date of colonisation: 2006
Genotype features TEP1*S1
Phenotype features:  susceptible to P. falciparum

Production protocol

Mosquitoes were reared according to standard procedures.

Product options

Please specify in your request:

Whole or dissected: 100 whole mosquitoes; or 50 dissected midguts, salivary glands or ovaries

Form of material:

  • Whole mosquitoes or dissected tissues, fixed in 70% ethanol, formalin, or other fixative: useful for DNA extraction from 70% ethanol, immunohistochemistry, not useful for RNA experiments or proteomics.
  • Whole mosquitoes or dissected tissues, homogenised in a lysis buffer such as Trizol: useful for RNA, DNA and protein experiments.
  • Whole mosquitoes or dissected tissues, frozen at -80°C or stored in RNALater useful for RNA, DNA and protein experiments.
  • Other formats or preparations may be possible by request, contact us.

Sample size: For larger sample sizes, request more units.

Publications

  • Harris, C., Lambrechts, L., Rousset, F., Abate, L., Nsango, S.E., Fontenille, D., Morlais, I., and Cohuet, A. (2010). Polymorphisms in Anopheles gambiae immune genes associated with natural resistance to Plasmodium falciparum. PLoS Pathog 6, e1001112.

For more information, please contact us.

Additional information

Provider country

France

Provider(s)

Institut Pasteur (IP)

Eligibility

In order to conform to H2020 rules promoting scientific interaction between countries, if your institute is located in the provider country, you need to choose another similar product, or form a user group to request this product, Please check: http://infravec2.eu/user-groups/