Description
Material provided: Whole mosquitoes, dissected tissues, or extracts
Unit definition: 100 mosquitoes (50 if dissected)
Vector information
Family: Culicidae
Genus: Anopheles
Species: Anopheles gambiae
Strain Name: KISUMU
Place of origin: Kisumu, Kenya
Date of colonisation: 1953
Production protocol
Eggs are hatched in dechlorinated tap water and larvae are reared under controlled conditions of 150–200 larvae per 1 liter and fed with one yeast tablet renewed every 3–4 days. Adults are kept in cages at 28°±1°C with a 16h: 8h light: dark cycle, 80% relative humidity, and supplied with a 10% sucrose solution.
Product options
Please specify in your request:
Sample size: For larger sample sizes, request more units.
Whole or dissected: 100 whole mosquitoes; or 50 dissected midguts, salivary glands or ovaries
Form of material:
- Whole mosquitoes or dissected tissues, fixed in 70% ethanol, formalin, or other fixative: useful for DNA extraction from 70% ethanol, immunohistochemistry, not useful for RNA experiments or proteomics.
- Whole mosquitoes or dissected tissues, homogenised in a lysis buffer such as Trizol: useful for RNA, DNA and protein experiments.
- Whole mosquitoes or dissected tissues, frozen at -80°C or stored in RNALater useful for RNA, DNA and protein experiments.
- Other formats or preparations may be possible by request, contact us.
For more information, please contact us