Description
The bioinformatic analysis service includes the following for each sample:
- Quality control and pre-processing of raw data
- Mapping to the genome or to the annotated transcriptome (when a reference sequence is available)
- Transcriptome assembly (if reference genome is not available or is incomplete)
- Differential gene expression analysis
- Gene Set enrichment analysis
- Data visualization including counts, PCA, volcano, and gene set enrichment dot plots (if required)
Material provided: data & bioinformatic analysis
Unit definition: Analysis of 800 million reads for 2×75 Paired End length (suggested at least 30 million of reads per sample – 24 samples per run).
1 Unit should allow testing at least four experimental conditions + two control conditions, with four replicates of each condition = total 24 RNA samples. Four replicates per condition are recommended. Please do not submit projects with less than three replicates per condition, which would be inadequate for statistical analysis.
END-USER RESPONSIBILITIES:
– DESPATCH OF DATA
- Bioinformatic data will be shared through HD or OneDrive platform
- The files should be labelled correctly and metadata information necessary for the analysis should be shared
- Complete the enclosed Sample Submission form and return by email to: customer@pologgb.com.
SUPPLIER RESPONSIBILITIES:
– STORAGE OF DATA
Polo d’Innovazione Genomica Genetica Biologia waives any responsibility for the storage and backup of the supplied project and sequencing data after delivery to the end-user. Polo d’Innovazione Genomica Genetica Biologia will store the project data only for 1 month after delivery. After expiration of this period, all data are removed from our servers and archived (unless otherwise agreed with the end-user). Your data might be re-imported at an extra cost within the period of 1 year after delivery of the final data. After 1 year, all project data will be deleted permanently from our systems. Extended data storage is available on request at an extra cost and must be requested at project start.
– DELIVERY OF RESULTS
The bioinformatics results will be sent according to the Modalities of Transfer with a Bioinformatic Reports PDF Files. The bioinformatic analysis will be executed in 45 days starting from the data sharing or from the generated sequencing data.
Modalities of transfer:
- by “WeTransfer” platform for data of up to 2Gb
- by “MegaSync” platform for data ranging from 2Gb to 15Gb
- by HD for data over 15 Gb
Please note that the cost of HD driver is not included. Where requested, the end-user must provide the HD.
For more information, please contact us.
For technical assistance, contact Polo GGB Technical Support:
Email: techsupport@pologgb.com
Telephone: 0039 0577 381310