Description
Material provided: Whole mosquitoes, dissected tissues, or extracts
Unit definition: 50 infected females
Description: User field-collected strain is sent to the Provider. 7-9 days old females are fed on an infectious blood-meal or intra-thoracically inoculated.
Vector information
Family: Culicidae
Genus: Culex
Species: Culex pipiens
Strain Name: UAB
Place of origin: Spain
Virus information
Pathogens used for infection: Usutu virus
Reference: USUV
ICTV Taxonomy: ssRNA+, Flaviviridae
Virus name: Usutu virus
Sequencing: partial sequence
Infectivity: Infectivity tested and quantified
Mycoplasmic content: Mycoplasma free
Production protocol
User field-collected strain is sent to the Provider. 7-9 days old females are fed on an infectious blood-meal using the Hemotek® system or intra-thoracically inoculated. The titer of infectious blood-meals is up to 107 TCID50/mL. Exposed females are transferred to small containers and fed with 10% sucrose in a chamber under controlled environmental conditions (temperature, light: dark cycle and humidity) until maximum day 14 post-exposure.
Product options
Please specify in your request:
Time post-infection: Please specify one time point in days post-infection (dpi) to harvest the samples. The most common options are primary midgut infection (3 dpi) or disseminated infection (7 or 14 dpi), but other times are possible by request, contact us.
Whole or dissected: Instead of 50 whole mosquitoes, material can be supplied from dissected material harvested at the appropriate time point: 25 dissected midguts or 25 dissected salivary glands or 25 dissected ovaries, provided with the corresponding carcasses.
Form of material: Whole mosquitoes or dissected tissues, fixed in formalin, or other fixative validated for virus inactivation: useful for DNA extraction, immunohistochemistry, not useful for RNA experiments or proteomics. Shipped as non-infectious material.
- Whole mosquitoes or dissected tissues, homogenised in a lysis buffer such as Trizol validated for virus inactivation: useful for RNA, DNA and protein experiments. Shipped as non-infectious material.
- Whole mosquitoes or dissected tissues, frozen at -80°C or stored in RNALater that do not efficiently inactivate virus: useful for RNA, DNA and protein experiments. Shipped as infectious material.
- Other formats or preparations may be possible by request, contact us.
Production conditions: Material is generated using default virus dose and incubation temperature used by the supplying facility. Other conditions may be possible upon request, contact us.
Sample size and replicates: For larger sample sizes, request more units. If requesting multiple units, please clearly specify the purpose (biological replicates, larger sample size, or other). Biological replicates (e.g., for RNAseq transcriptional profiling experiments) require independent infections, while a larger sample size of the same material requires infecting additional mosquitoes on the same infectious blood.
For more information, please contact us.