Description
Material provided: Live eggs
Unit definition: 10 egg rafts (each raft contains 150-200 eggs)
Vector information
Family: Culicidae
Genus: Culex
Species: Culex quinquefasciatus
Strain Name: Slab
Place of origin: California, USA
Date of colonisation: 1966 (Georghiou et al)
Production protocol
Eggs are laid and hatched in dechlorinated tap water and larvae are reared under controlled conditions of 300-400 larvae per litre. Larvae are fed every 3 days with 200 mg of a 1:2 mix of Tetramin®/ powdered rabbit food. Larval tray water is changed on feeding days to avoid bacterial growth on the water surface. When pupation starts, trays are placed inside an emergence cage (at 26°±1°C with a 12h:12h light: dark cycle, 70% relative humidity) and provided with an ad libitum source of 10% sugar solution for the emerged adults.
Publications
Georghiou GP, Metcalf RL and Gidden FE (1966) Carbamate-resistance in mosquitoes: selection of Culex pipiens fatigans Wied. for resistance to Baygon. Bull. WHO 35: 691-708
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