Description
Material provided: Whole mosquitoes, dissected tissues, or extracts
Unit definition: 100 mosquitoes (50 if dissected)
Vector information
Family: Culicidae
Genus: Culex
Species: Culex quinquefasciatus
Strain Name: Slab
Place of origin: California, USA
Date of colonisation: 1966 (Georghiou et al)
Production protocol
Eggs are laid and hatched in dechlorinated tap water and larvae are reared under controlled conditions of 300-400 larvae per litre. Larvae are fed every 3 days with 200 mg of a 1:2 mix of Tetramin®/ powdered rabbit food. Larval tray water is changed on feeding days to avoid bacterial growth on the water surface. When pupation starts, trays are placed inside an emergence cage (at 26°±1°C with a 12h:12h light: dark cycle, 70% relative humidity) and provided with an ad libitum source of 10% sugar solution for the emerged adults.
Product options
Please specify in your request:
Sample size: For larger sample sizes, request more units.
Whole or dissected: 100 whole mosquitoes; or 50 dissected midguts
Form of material:
- Whole mosquitoes or dissected tissues, fixed in 70% ethanol, formalin, or other fixative: useful for DNA extraction from 70% ethanol, immunohistochemistry, not useful for RNA experiments or proteomics.
- Whole mosquitoes or dissected tissues, homogenised in a lysis buffer such as Trizol: useful for RNA, DNA and protein experiments.
- Whole mosquitoes or dissected tissues, frozen at -80°C or stored in RNALater useful for RNA, DNA and protein experiments.
Other formats or preparations may be possible by request, contact us.
Publications
Georghiou GP, Metcalf RL and Gidden FE (1966) Carbamate-resistance in mosquitoes: selection of Culex pipiens fatigans Wied. for resistance to Baygon. Bull. WHO 35: 691-708
For more information, please contact us.